Journal: Genes

Article Title: Optimizing Analytical Thresholds for Low-Template DNA Analysis: Insights from Multi-Laboratory Negative Controls.

doi: 10.3390/genes15010117

Figure Lengend Snippet: Figure 5. The variation in allele dropouts and non-allele peaks across five dyes for low-template control DNA 9947A detected using the VeriFiler™Plus kit in LAB_a. The AT was systematically adjusted from 1 to 200 RFU.

Article Snippet: Female control DNA 9947A (OriGene, Rockville, MD, USA) was diluted to three concentrations: 31.25 pg/μL, 15.625 pg/μL, and 7.8125 pg/μL.

Techniques: Control

Journal: Science immunology

Article Title: Human Innate Lymphoid Cell Precursors Express CD48 that Modulates ILC Differentiation through 2B4 Signaling

doi: 10.1126/sciimmunol.aay4218

Figure Lengend Snippet: (A) Freshly isolated UCB-derived CD34+ HSCs were stained for CD244 and CD48 (representative, n=4). (B) CD244 in CD34+CD48− cells (aqua) and in CD34+CD48+ cells (red) for day 1 and day 3 UCB-derived CD34+ HSCs (representative, n=4). (C) SAP mRNA expression in day 5 CD34+CD48− and CD34+CD48+ cells (n=4/group). (D and E) Anti-CD244 and anti-CD48 blocking antibodies or isotype IgG controls? were added to differentiating CD34+α4β7+CD48+ cultures to block CD244 signaling. (F and G) CD34+α4β7+CD48+ cells were plated on anti-CD244 or IgG coated plates twice for 4 days prior to 21 days of differentiation to activate CD244 signaling via cross-linking. Cells were stained for ILCs, and the percentage (D and F) or absolute number (E and G) of CD94+ NK cells, CD294+ ILC2 and CD117+ ILC3 are shown (n=4/group). (H) CD244+ cultures are shown for cells transfected with control or CD244 gRNA (n=3/group). (I) Representative ILC2 (CD294) staining for cultures transfected with control or CD244 gRNA. (J) Dot plot showing generation of ILC2s from cultures that express (control gRNA) or lack (CD244 gRNA) CD244. (C-H) Mean ± SD, paired t-tests (C, F, G and H) or one-way ANOVA (D and E) and p-values are depicted.

Article Snippet: The Alt-R Cas9 Nuclease V3, universal tracrRNA, CD244 gRNA and non-human control gRNA were purchased from Integrated DNA Technologies.

Techniques: Isolation, Derivative Assay, Staining, Expressing, Blocking Assay, Transfection